Meu Carrinho

0 ITEM(s)

TOTAL: R$ 0,00

IR PARA O CARRINHO

sinapse@sinapsebiotecnologia.com.br

(11) 2605-5655

Ligue grátis: 0800 006 5655 (exceto São Paulo capital e celulares)

ESCOLHA A OPÇÃO BOLETO PARA PAGAR SÓ DEPOIS DO RECEBIMENTO
NAS NOTAS PARA A FAPESP A ISENÇÃO DE ICMS SERÁ CONFIRMADA POR E-MAIL
INFORME DADOS DO PROJETO NO CAMPO DE OBSERVAÇÕES DO PEDIDO

 

O valor do frete será somado ao valor dos itens e não estará descrito na nota fiscal.

 

cDNA / RT-PCR

Kits para Síntese de cDNA

Maxima™ H Minus cDNA Synthesis Master Mix, with dsDNase - Thermo Scientific - K1681

Maxima™ H Minus cDNA Synthesis Master Mix, with dsDNase - Thermo Scientific - K1681

(K1681)

Marca:  Thermo Scientific  | 

Embalagem:  20 reactions

KIT PARA CDNA DE ALTA EFICIÊNCIA COM REMOÇÃO DE DNA GENOMICO POR DSDNASE

Prazo de Entrega: 60-90 dias corridos

mais informações

Maxima™ H Minus cDNA Synthesis Master Mix, with dsDNase - Thermo Scientific - K1681

(K1681)

Marca:  Thermo Scientific  | 

Embalagem:  20 reactions

KIT PARA CDNA DE ALTA EFICIÊNCIA COM REMOÇÃO DE DNA GENOMICO POR DSDNASE

Prazo de Entrega: 60-90 dias corridos

mais informações

As combinações acima não possuem estoque.

Preço sob consulta

Consulte nosso preço

Descrição do Produto

Maxima™ H Minus cDNA Synthesis Master Mix, with dsDNase

 

Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit with dsDNase provides a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure. The kit contains a novel double-strand specific DNase (dsDNase) engineered to remove contaminating genomic DNA from RNA preps in 2 minutes without damage to quality or quantity of RNA. Highly specific dsDNase activity towards double-stranded DNA ensures that single-stranded DNA (such as cDNA and primers) is not cleaved and dsDNase treated RNA can be directly added to reverse transcription.

For reverse transcription the kit uses Thermo Scientific Maxima H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by in vitro evolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65°C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.

 

Highlights

• Integrated genomic DNA removal step
• Increased reaction temperatures—the first strand of cDNA can be synthesized within the 42 to 65°C temperature range
• High yields of full-length first strand cDNA—with RNA templates up to 20 kb
• Flexible priming—oligo(dT)18, random hexamer, or gene-specific primers

 

Applications

• First Strand cDNA synthesis for RT-PCR
• Construction of cDNA libraries
• Generation of probes for hybridization
• Antisense RNA synthesis

 

Includes

Maxima H Minus First Strand cDNA Synthesis Kit with dsDNase contains Maxima H Minus Enzyme Mix, dsDNase, 10X dsDNase Buffer, Oligo(dT)18 and Random hexamer primers, 5X RT Buffer, dNTP Mix, and nuclease-free water.

 

Additional information about reaction components

• Maxima H Minus Enzyme Mix contains Maxima H Minus Reverse Transcriptase and RiboLock RNase Inhibitor. RiboLock RNase Inhibitor effectively protects RNA templates from degradation by RNases A, B and C at temperatures up to 55°C.
• oligo(dT)18 and random hexamer primers are supplied with the kit. Random hexamer primers bind non-specifically and are used to synthesize cDNA from all RNAs in a total RNA population. The oligo(dT)18 primer selectively anneals to the 3'-end of poly(A) RNA, synthesizing cDNA only from poly(A) tailed mRNA. Gene-specific primers may also be used with the kit to prime synthesis from a specified sequence.
• 10 mM dNTP Mix is a premixed aqueous solution of dATP, dTTP, dCTP, and dGTP.
• Nuclease-free water is provided for reaction set-up and dilution of sample DNA. The absence of endo-, exodeoxyribonucleases, ribonucleases, and phosphatases has been confirmed by appropriate quality tests.

 

PRODUTOS RELACIONADOS

Produtos Relacionados

Sinapse Biotecnologia e Cookies: Nós utilizamos cookies para personalizar anúncios e melhorar a sua experiência na loja. Ao continuar navegando, você concorda com a nossa Política de Privacidade.

Continuar e fechar