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Purificação de PCR e Eletroforese
illustra GFX PCR DNA and Gel Band Purification Kit, 250 Purifications - Cytiva - 28903471
(28903471)
Marca:  Cytiva  | 
Embalagem:  250 Purifications
REAGENTES DE PURIFICACAO GFX PCR DNA GEL BAND (250 PURIF.)
Prazo de Entrega: 5-10 dias úteis
mais informações
(28903471)
Marca:  Cytiva  | 
Embalagem:  250 Purifications
REAGENTES DE PURIFICACAO GFX PCR DNA GEL BAND (250 PURIF.)
Prazo de Entrega: 5-10 dias úteis
mais informações
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Designed for the rapid purification and concentration of PCR products or DNA fragments ranging in size from 50 bp to 10 kb. This kit can be used to purify DNA from reaction volumes up to 100 μl or agarose gel slices up to 900 mg.
illustra GFX PCR DNA and Gel Band Purification Kit is designed for the rapid purification and concentration of PCR products or DNA fragments ranging in size from 50 bp to 10 kb. This kit can be used to purify DNA from reaction volumes up to 100 μl or agarose gel slices up to 900 mg. The kit combines a versatile chaotropic buffer with a glass fiber matrix supported in a spin column for the purification of DNA from both solution and agarose gel. Typical recoveries range from 60% to 80% for DNA fragments from agarose gel to as high as 95% for PCR products from solution. DNA purity is exceptional; 99.5% of contaminants are removed.
illustra GFX PCR DNA and Gel Band Purification Kit contains the following components in sufficient quantities: GFX columns, collection tubes, color-coded bottles of capture buffer, wash buffer, and two elution buffers (Tris-HCl and sterile water), and an instruction booklet.
Parameter | illustra GFX PCR DNA and Gel Band Purification Kit, 250 purifications |
---|---|
Sample Type | PCR mixtures, enzyme reactions, DNA solutions, agarose gel slices |
Sample Size | 50 bp-10 kb |
Input Volume | 100 µl solution or up to 900 mg agarose |
Elution Volume | 10–50 µl (into one of two elution buffers provided) |
Yield | PCR 10 µl elution volume-65%, PCR 50 µl elution volume-82%, 300 mg agarose 10 µl elution volume-57%, 300 mg agarose 50 µl elution volume-91% |
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