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PCR Convencional

Reagentes

Water, nuclease-free

Water, Molecular Biology Grade, DNase, RNase, Protease free - GE Healthcare - SH30538.01

Water, Molecular Biology Grade, DNase, RNase, Protease free - GE Healthcare - SH30538.01

(SH30538.01)

Marca:  GE Healthcare - Hyclone  | 

Embalagem:  100 mL

Prazo de Entrega: 30-40 dias corridos

mais informações

Water, Molecular Biology Grade, DNase, RNase, Protease free - GE Healthcare - SH30538.01

(SH30538.01)

Marca:  GE Healthcare - Hyclone  | 

Embalagem:  100 mL

Prazo de Entrega: 30-40 dias corridos

mais informações

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por: R$ 54,87

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Descrição do Produto

HyClone™ Water, Molecular Biology Grade

 

For critical applications involving nucleotides such as RNA and DNA, use nuclease-free GE Healthcare HyClone Molecular Biology-Grade Water, containing no DEPC or other carcinogenic chemicals.

 

 

Molecular Biology Grade Water - 100 mL

 

Item Description HyPure Molecular Biology Grade Water, deionized, distilled; no detectable nucleases or proteases
pH 5.0 to 7.0
Format Liquid
Quantity 100 mL
Storage Requirements 2° to 30° C
Shelf Life 24 months
Endotoxin Concentration ≤ 0.05 EU/mL
Sterilization 0.1 um sterile filtered
Certifications/Compliance Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities

 

Product Overview

 

Quality:

Nuclease-free water is certified to have no detectable contaminating activity in DNase, RNase, and protease assays

Maximum allowable limit of endotoxin is 0.05 EU/mL

Purification process includes continuous deionization, reverse osmosis, ultraviolet light treatment, distillation, and 0.1µm filtration

 

Applications:

Buffer preparation

DNA/RNA purification

DNA sequencing and libraries

Gene mapping

Recombinant DNA and cloning

PCR analysis

Northern Blot

Southern Blot

 

 

FOR FURTHER MANUFACTURING OR RESEARCH USE.  NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.

 

In order to work with nucleotides such as RNA and DNA and to prevent subsequent degradation due to enzymatic activity, it is necessary to use reagents, including water, that are devoid of all nucleases. Nucleases are naturally occurring enzymes whose role is specifically to cleave nucleotide polymeric molecules such as DNA or RNA at various points. Theoretically, just one molecule of a nuclease could systematically facilitate the destruction of an entire nucleotide sample over time.