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NAS NOTAS PARA A FAPESP A ISENÇÃO DE ICMS SERÁ CONFIRMADA POR E-MAIL
INFORME DADOS DO PROJETO NO CAMPO DE OBSERVAÇÕES DO PEDIDO

 

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Epigenética

Metilação

Outros

Human HCT116 DKO Non-methylated DNA - Zymo Research - D5014-1

Human HCT116 DKO Non-methylated DNA - Zymo Research - D5014-1

(D5014-1)

Marca:  Zymo Research  | 

Embalagem:  20 ul

PADRÃO DE DNA NÃO METILADO

Prazo de Entrega: 60-90 dias corridos

mais informações

Human HCT116 DKO Non-methylated DNA - Zymo Research - D5014-1

(D5014-1)

Marca:  Zymo Research  | 

Embalagem:  20 ul

PADRÃO DE DNA NÃO METILADO

Prazo de Entrega: 60-90 dias corridos

mais informações

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Descrição do Produto

Human Methylated & Non-methylated DNA Set
 

Purified, non-methylated and methylated human DNA (5 µg/20 µl of each) for use as negative and positive control in methylation detection applications
Set of control primers designed to amplify non-methylated, methylated, and mixed methylation copies of the death-associated protein kinase 1 gene (DAPK1) following bisulfite conversion.

 

About Human Methylated & Non-methylated DNA Set

The Human Methylated & Non-methylated DNA Set consists of two control DNAs (a methylated human DNA standard and a non-methylated human DNA standard) together with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation™, EZ DNA Methylation-Gold™, and EZ DNA Methylation-Direct™ kits from Zymo Research to assess the efficiency of bisulfite-mediated conversion of DNA. The non-methylated human DNA is purified from the HCT116 DKO (double knock-out) cell line, which contains genetic knockouts of both DNA methyltransferases DNMT1 (-/-) and DNMT3b (-/-). The DNA derived from HCT116 DKO cells has a low level of DNA methylation and therefore can be used as a negative control for DNA methylation analysis (see below). The methylated human DNA standard is purified HCT116 DKO DNA that has been enzymatically methylated at all cytosine positions comprising CG dinucleotides by CpG Methylase and can be used as a positive control for DNA methylation analysis.
 

Link do Produto: http://www.zymoresearch.com/epigenetics/dna-methylation/methylated-dna-standards/human-methylated-non-methylated-dna-set

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