Meu Carrinho

0 ITEM(s)

TOTAL: R$ 0,00

IR PARA O CARRINHO

sinapse@sinapsebiotecnologia.com.br

(11) 2605-5655

Ligue grátis: 0800 006 5655 (exceto São Paulo capital e celulares)

ESCOLHA A OPÇÃO BOLETO PARA PAGAR SÓ DEPOIS DO RECEBIMENTO
NAS NOTAS PARA A FAPESP A ISENÇÃO DE ICMS SERÁ CONFIRMADA POR E-MAIL
INFORME DADOS DO PROJETO NO CAMPO DE OBSERVAÇÕES DO PEDIDO

 

O valor do frete será somado ao valor dos itens e não estará descrito na nota fiscal.

 

Clonagem

Enzimas para Clonagem

Restrição

FastDigest StyI (Eco130I) - Thermo Scientific - FD0414

FastDigest StyI (Eco130I) - Thermo Scientific - FD0414

(FD0414)

Marca:  Thermo Scientific  | 

Embalagem:  200 rxns

ENZIMA DE RESTRICAO DE ACAO RAPIDA STYI , 200 REACOES

Prazo de Entrega: 60-90 dias corridos

mais informações

FastDigest StyI (Eco130I) - Thermo Scientific - FD0414

(FD0414)

Marca:  Thermo Scientific  | 

Embalagem:  200 rxns

ENZIMA DE RESTRICAO DE ACAO RAPIDA STYI , 200 REACOES

Prazo de Entrega: 60-90 dias corridos

mais informações

As combinações acima não possuem estoque.

Preço sob consulta

Consulte nosso preço

Descrição do Produto

FastDigest Eco130I

5'

 

 

C ↓

C  

W  

W  

G  

G  

 

3'

 

3'

 

 

G  

G  

W  

W  

C ↑

C  

 

5'

 


Thermo Scientific FastDigest Eco130I restriction enzyme recognizes C^CWWGG site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: StyI, BssT1I, EcoT14I, ErhI.

Thermo Scientific FastDigest Eco130I is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. SeeReaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow FragmentT4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features

• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes available

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysis

Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity, refer to product specifications.

PRODUTOS RELACIONADOS

Produtos Relacionados

Sinapse Biotecnologia e Cookies: Nós utilizamos cookies para personalizar anúncios e melhorar a sua experiência na loja. Ao continuar navegando, você concorda com a nossa Política de Privacidade.

Continuar e fechar