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EZ-Link HPDP-Biotin - Thermo Scientific - 21341

EZ-Link HPDP-Biotin - Thermo Scientific - 21341

(21341)

Marca:  Thermo Scientific  | 

Embalagem:  50 MG

Prazo de Entrega: 30-40 dias corridos

mais informações

EZ-Link HPDP-Biotin - Thermo Scientific - 21341

(21341)

Marca:  Thermo Scientific  | 

Embalagem:  50 MG

Prazo de Entrega: 30-40 dias corridos

mais informações

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Descrição do Produto

EZ-Link™ HPDP-Biotin


In proteins, sulfhydryls exist where there are cysteine (C) residues. Cystine disulfide bonds must be reduced to make sulfhydryl groups available for labeling. Hinge-region disulfide bridges of antibodies can be selectively reduced to make functional half-antibodies that can be labeled.

 are available: maleimido, iodoacetyl and pyridyldithiol. Pyridyldithiol reagents specifically react with sulfhydryl groups (-SH) in near-neutral buffers to form reversible disulfide bonds.types of sulfhydryl-reactive compounds differ in reactivity, length, solubility, cell permeability and cleavability. Three Biotinylation reagents

We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.

HPDP-Biotin is a pyridyldithiol-biotin compound for labeling protein cysteines and other molecules that contain sulfhydryl groups. This reagent specifically reacts with reduced thiols (-SH) in near-neutral buffers to form reversible disulfide bonds. HPDP-Biotin is useful for labeling and affinity-purification applications that require recovery of the original, unmodified molecule. For example, a protein can be biotinylated, allowed to bind its interactor, then captured to a streptavidin column and finally eluted and recovered by reduction of the disulfide bond with dithiothreitol.

—spacer arm (total length added to target) is 29.2 angstromsMedium length
—must be dissolved in DMSO or DMF before further dilution in aqueous buffersSolubility
—forms disulfide bonds, which can be cleaved using DTT or other reducing agentReversible
—perform reactions at pH 6.5 to 7.5 in buffers such as PBSPyridyldithiol-activated
—reacts with sulfhydryls (-SH), such as the side-chain of cysteine (C)Thiol-reactive
—biotinylate antibodies or other proteins for use in protein methodsProtein labeling
Features of EZ-Link HPDP-Biotin:

Thermo Scientific EZ-Link HPDP-Biotin is pyridyldithiol-activated, sulfhydryl-reactive biotinylation reagent that conjugates via a cleavable (reversible) disulfide bond to enable use in a variety of purification methods.

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