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Sequenciamento

NGS

Quantificação De Biblioteca Para Illumina

DNA Standards for Library Quantification - Takara Bio - 638325

DNA Standards for Library Quantification - Takara Bio - 638325

(638325)

Marca:  Takara Bio  | 

Embalagem:  50 Rxns

Prazo de Entrega: 60-90 dias corridos

mais informações

DNA Standards for Library Quantification - Takara Bio - 638325

(638325)

Marca:  Takara Bio  | 

Embalagem:  50 Rxns

Prazo de Entrega: 60-90 dias corridos

mais informações

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por: R$ 3.675,00

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Descrição do Produto

Link do Produto: http://www.clontech.com/US/Products/cDNA_Synthesis_and_Library_Construction/Next_Gen_Sequencing_Kits/Library_Quantification_Kit

 

NGS Library Quantification Kit

 

To obtain high-quality Illumina® next-generation sequencing (NGS) data, loading the flow cell with an appropriate amount of library DNA is essential. Loading an insufficient amount of library DNA will result in low cluster density and reduced sequencing yield; an overabundance of library DNA may increase cluster density and result in poor quality data. Standard methods of NGS library quantification, such as those based on electrophoresis or spectrophotometry, have low sensitivity, are non-specific for adapter-bound DNA, and typically require a large amount of library sample for analysis. The Library Quantification Kit provides a highly-sensitive, qPCR-based quantification method that specifically targets adapter sequences used for Illumina’s NGS reactions. This kit allows specific measurement of the sequencing library concentration even in samples that contain DNA molecules not bound to adapters. This kit can also be used to confirm adapter ligation during library preparation.

 

Library quantification with this kit is accomplished by qPCR using SYBR® Green for detection. Amplification products are detected by intercalation of SYBR Green I in double-stranded DNA. Fluorescence detection in real time allows quantification of amplification products. This kit includes DNA standards of known concentration to allow absolute quantification of library DNA.

 

Features

 

  • Highly sensitive quantification of Illumina sequencing libraries—the qPCR-based assay can measure even low concentration libraries
  • Allows specific quantification of only DNA molecules bound to adapters
  • Reduces variability in cluster density to improve sequencing results—use prior to sequencing to ensure optimal loading concentration
  • Complete kit—includes qPCR primers and reagents, and DNA standards for absolute quantification using a standard curve

 

Applications

 

  • Illumina NGS library quantification by qPCR

 

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