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Sequenciamento

NGS

Kits para RNA-Seq

SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian - Takara Bio - 634876

SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian - Takara Bio - 634876

(634876)

Marca:  Takara Bio  | 

Embalagem:  96 Rxns

Prazo de Entrega: 30-60 dias corridos

mais informações

SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian - Takara Bio - 634876

(634876)

Marca:  Takara Bio  | 

Embalagem:  96 Rxns

Prazo de Entrega: 30-60 dias corridos

mais informações

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Descrição do Produto

Link do Produto: http://www.clontech.com/AU/Products/cDNA_Synthesis_and_Library_Construction/Next_Gen_Sequencing_Kits/Total_RNA-Seq/High_Input_Total_RNA_Seq_Illumina

 

SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian—Strand-Specific RNA-Seq Libraries for Illumina Platforms from High-Input Total RNA

 

The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian provides a rapid solution for preparing indexed Illumina® sequencing libraries from 100 ng–1 µg of mammalian total RNA. This kit combines key RiboGone and SMART technologies, seamlessly blending efficient rRNA removal and strand-specific library generation in around 5 hours. Because rRNA comprises ~90% of total RNA, effective depletion of these abundant transcripts prior to library generation can lower sequencing costs and improve mapping statistics. The core SMART technology completes a streamlined process that maintains strand information, and eliminates downstream library prep by incorporating indexes and adapters during the reverse transcription and PCR amplification steps.

 

These high-input kits contain 12 Reverse Primers and up to 8 Forward Primers to generate up to 96 separate indexes in HT format. The specific primers you receive will depend on your particular kit. The entire protocol—from rRNA depletion through creation of Illumina-specific, indexed RNA-seq libraries—takes only ~5 hours.

 

For lower input amounts (e.g., 100 pg–100 ng) of input total RNA, we recommend using the SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian.

 

Features

 

  • Strand information—Identify each transcript’s strand of origin with >99% accuracy.
  • Fast, streamlined protocol—Go from start to finish in ~5 hours.
  • Large input size—Use 100 ng–1 µg total RNA from human, mouse, or rat.
  • Versatility—Get reliable data across replicates and RNA quality. See high-quality, reproducible data from total RNA with a RIN (RNA Integrity Number) from 3–10.
  • Integrated with Illumina sequencing—Generate Illumina-specific libraries with up to 96 indexes, and retain strand information.

 

Applications

 

  • Illumina-specific NGS library generation that retains strand information
  • RNA-Seq on Illumina platforms for mammalian samples
  • Coding and non-coding transcript information

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