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cDNA / RT-PCR

Kits para Síntese de cDNA

RACE

SMARTer® RACE 5’/3’ Kit - 20 Rxns - 634859 - Takara Bio

SMARTer® RACE 5’/3’ Kit - 20 Rxns - 634859 - Takara Bio

(634859)

Marca:  Takara Bio  | 

Embalagem:  20 Rxns

KIT DE AMPLIFICACAO DE CDNA PARA AMPLIFICACAO RAPIDA DE CDNA E ENDS-5'RACE E 3'RACE PARA OBTER A SEQUENCIA COMPLETA DE UMA TRANSCRICAO DE RNA, 20 REACOES

Prazo de Entrega: 30-60 dias corridos

mais informações

SMARTer® RACE 5’/3’ Kit - 20 Rxns - 634859 - Takara Bio

(634859)

Marca:  Takara Bio  | 

Embalagem:  20 Rxns

KIT DE AMPLIFICACAO DE CDNA PARA AMPLIFICACAO RAPIDA DE CDNA E ENDS-5'RACE E 3'RACE PARA OBTER A SEQUENCIA COMPLETA DE UMA TRANSCRICAO DE RNA, 20 REACOES

Prazo de Entrega: 30-60 dias corridos

mais informações

As combinações acima não possuem estoque.

por: R$ 12.321,38

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Descrição do Produto

Link do Produto: http://www.clontech.com/BR/Products/cDNA_Synthesis_and_Library_Construction/RACE_Rapid_Amplification_of_cDNA_Ends/5_Prime_RACE_and_3_Prime_RACE?sitex=10028:22372:US

 

cDNA Amplification Kits for Rapid Amplification of cDNA Ends—5' RACE & 3' RACE

 

Rapid Amplification of cDNA Ends (RACE) is a technique used to obtain the full-length sequence of an RNA transcript. RACE can provide the sequence of an RNA transcript from a small known sequence within the transcript all the way to the 5' end (5' RACE-PCR) or 3' end (3' RACE-PCR) of the RNA. 

 

The SMARTer RACE 5’/3’ Kit is an improved version of the SMARTer RACE cDNA Amplification Kit. The SMARTer RACE 5’/3’ Kit provides improved sensitivity, less background and higher specificity over the previous generation of kits. The first-strand cDNA generated with this kit can be used directly in 5’- and 3’-RACE PCR reactions. The SMARTer protocol does not involve any of the adaptor ligation steps that other RACE kits incorporate, making the protocol shorter and significantly easier to use. The new kit is also designed to accommodate larger RNA input volumes (up to 11 µl) and has been shown to perform more efficiently on challenging targets (e.g., those that are long, GC-rich, etc.). The kit includes our highly robust SeqAmp DNA Polymerase for amplification of products, and the In-Fusion HD Cloning Kit for efficient cloning of RACE fragments in the provided linearized vector. The SMARTer RACE 5’/3’ procedure combines SMART first-strand cDNA synthesis technology with a powerful suppression PCR protocol that vastly reduces the background amplification commonly associated with RACE protocols.

 

Features

 

SMARTer RACE 5'/3' Kit

 

  • Simple kit—easy to use
  • SeqAmp DNA Polymerase provides robust PCR performance
  • In-Fusion HD technology enables fast, easy cloning of RACE fragments
  • Requires Only 10 ng of total RNA
  • Specific enrichment for either 5' or 3' ends
  • No RNA pretreatment required

 

Marathon cDNA Amplification Kit

 

  • Quickly obtain cDNAs without library screening
  • Avoid troublesome single-stranded ligation or tailing reactions
  • Perform 5' and 3' RACE from the same template

 

Applications

 

  • RACE (Rapid Amplification of cDNA Ends)
  • Identification of 3'/5' UTRs
  • Full-length gene cloning
  • Gene cloning and analysis

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