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Extração de Ácidos Nucleicos

Extração de DNA

DNA de micro-organismos

illustra DNA bacteria genomicPrep Mini Spin Kit, 250 reactions - GE Healthcare - 28904259

illustra DNA bacteria genomicPrep Mini Spin Kit, 250 reactions - GE Healthcare - 28904259

(28904259)

Marca:  GE Healthcare Life Sciences  | 

Embalagem:  250 reactions

REAGENTES DE PURIFICACAO DNA ILLUSTRA BACTERIA MINI (250REC)

Prazo de Entrega: 40-60 dias corridos

mais informações

illustra DNA bacteria genomicPrep Mini Spin Kit, 250 reactions - GE Healthcare - 28904259

(28904259)

Marca:  GE Healthcare Life Sciences  | 

Embalagem:  250 reactions

REAGENTES DE PURIFICACAO DNA ILLUSTRA BACTERIA MINI (250REC)

Prazo de Entrega: 40-60 dias corridos

mais informações

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por: R$ 7.292,73

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Descrição do Produto

illustra bacteria genomicPrep Mini Spin Kit

 

illustra bacteria genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from Gram-negative (G-) and Gram-positive (G+) bacteria.

 

  • For the rapid extraction and purification of high-quality genomic DNA from gram-positive and gram-negative bacteria.
  • Total DNA extraction time under 1 h with reduced lysis time.
  • Can be used to purify 4 to 8 μg of genomic DNA from 2 × 10? bacteria cells with input amounts ranging from 1 × 10? to 4 × 10? bacteria cells.
  • Spin columns, prepacked with a silica membrane, provide excellent reproducibility and consistency.
  • Verified in several downstream applications including RT PCR, long PCR, and restriction enzyme digests.
  • Color-coded for ease of use.

 

illustra bacteria genomicPrep Mini Spin Kit can be used to isolate genomic DNA from a wide range of bacterial strains such as DH5α, TOP10, JM109, Bacillus subtilis, etc. The kit produces highly pure and intact genomic DNA with A260/A280values of 1.75 to 1.85. DNA at concentrations suitable for most downstream applications including restriction enzyme digestion, RT-PCR, and long PCR. The method uses a lysis solution in combination with proteinase K to gently release genomic DNA into solution from bacteria cells. The DNA is deproteinated in the extraction solution and the genomic DNA is then bound onto a silica column in the presence of chaotropic solution. Contaminants are removed during wash steps and the purifed DNA is eluted with prewarmed TE solution.