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Estudo de Proteínas

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Colunas de Níquel

HisTrap HP protein purification columns, 5 x 1 ml - GE Healthcare - 17524701

HisTrap HP protein purification columns, 5 x 1 ml - GE Healthcare - 17524701

(17524701)

Marca:  GE Healthcare Life Sciences  | 

Embalagem:  5 x 1 ml

COLUNA CROMATOGRAFICA HISTRAP SEPHAROSE HP (5X 1 ML)

Prazo de Entrega: 90-120 dias corridos

mais informações

HisTrap HP protein purification columns, 5 x 1 ml - GE Healthcare - 17524701

(17524701)

Marca:  GE Healthcare Life Sciences  | 

Embalagem:  5 x 1 ml

COLUNA CROMATOGRAFICA HISTRAP SEPHAROSE HP (5X 1 ML)

Prazo de Entrega: 90-120 dias corridos

mais informações

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Descrição do Produto

HisTrap HP histidine-tagged protein purification columns


HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.

  • High resolution – due to small bead size (average bead size is 34 µm).
  • High binding capacity for high protein recovery – exceeds 40 mg histidine-tagged (his-tagged) protein/mL resin.
  • Broad reagent compatibility – compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
  • Convenient HiTrap format: Compatible with syringe, pump, or chromatography system such as ÄKTA systems or other FPLC systems.


His-tag protein purification columns

HisTrap HP columns are packed with Ni Sepharose High Performance (HP) affinity resin. This resin consists of highly cross-linked agarose beads to which a chelating group has been coupled. The chelating group is precharged with nickel, which selectively retains proteins with exposed histidine groups.

This IMAC resin has a binding capacity that exceeds 40 mg/mL, resulting in high protein recovery. The small bead size contributes to a more efficient purification compared to nickel resins of larger bead size.


Successful use in published research

HisTrap HP columns are used in a variety of applications, including:

 

Other GE products for his-tag protein purification

GE Healthcare provides a large variety of Life Sciences solutions for IMAC protein purification. For high binding capacity or when scalability is needed, consider HisTrap FF Crude columns.