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Estudo de Proteínas

Purificação de Proteínas

Colunas de Cobalto

Histalon™ Gravity Column Purification Kit - Takara Bio - 635654

Histalon™ Gravity Column Purification Kit - Takara Bio - 635654

(635654)

Marca:  Takara Bio  | 

Embalagem:  5 x 1 mL Columns

COLUNAS PARA PURIFICACAO DE PROTEINAS COM HIS, 5 COLUNAS

Prazo de Entrega: 30-60 dias corridos

mais informações

Histalon™ Gravity Column Purification Kit - Takara Bio - 635654

(635654)

Marca:  Takara Bio  | 

Embalagem:  5 x 1 mL Columns

COLUNAS PARA PURIFICACAO DE PROTEINAS COM HIS, 5 COLUNAS

Prazo de Entrega: 30-60 dias corridos

mais informações

As combinações acima não possuem estoque.

por: R$ 4.010,63

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Descrição do Produto

Link do Produto: http://www.clontech.com/BR/Products/Protein_Expression_and_Purification/His-Tagged_Protein_Purification/Cobalt_Columns-Gravity?sitex=10028:22372:US

 

HisTALON Gravity Columns—Fast, Easy His-Tagged Protein Purification

 

TALON Metal Affinity Resin is a durable immobilized metal affinity chromatography (IMAC) resin that has a remarkable affinity and specificity for his-tagged proteins. TALON Resin is compatible with all commonly used IMAC reagents and allows protein purification under native or denaturing conditions.
TALON Metal Affinity Resin uses cobalt ions for purifying recombinant his-tagged proteins. TALON is a tetradentate chelator charged with cobalt, and is specific for his-tagged proteins.
Clontech offers ready-to-use HisTALON Gravity Columns for the efficient purification of his-tagged proteins from bacterial, mammalian, and baculovirus-infected cells, using gravity-flow-based protocols. The columns are prepacked with our TALON Resin and can absorb more than 20 mg of his-tagged AcGFP1. These columns enable fast, easy, and reproducible chromatographic separation—and can be regenerated for multiple uses. However, we recommend that you reuse a column only to purify different batches of the same protein. If you plan to purify multiple proteins using the same column, you must utilize the “Complete Regeneration” method described in the User Manual.

 

Use of Reducing Agents


Purification with TALON Resin may be carried out in the presence of ?-mercaptoethanol (see example), but not DTT or DTE, to preserve reduced sulfhydryl (-SH) groups that are important for the biological activity and structure of a given protein. TALON provides higher yields than Ni-NTA (see example) in the presence of ?-mercaptoethanol.

 

Features

 

• Easy-to-use prepacked gravity columns
• Maximize yield of biologically active protein
• Superior purity & high yields with TALON Resin

 

Applications

 

• Crystallography
• Functional assays
• Structural investigations
• Other applications

 

Reagents Incompatible with TALON Resin

 

• DTT (dithiothreitol) and DTE (dithioerythritol)
NOTE: Using strong reducing agents will interfere with cobalt metal ion binding to the resin.
• EDTA (ethylenediaminetetraacetic acid), EGTA (ethylene glycol tetraacetic acid), and PEI
NOTE: Although you can use EDTA at indicated points, it must be removed from the sample by gel filtration prior to applying the sample to TALON Resins.

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