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Silenciamento e Edição Gênica

CRISPR/CAS9

Guide-it™ CRISPR/Cas9 System (Red) - Takara Bio - 632602

Guide-it™ CRISPR/Cas9 System (Red) - Takara Bio - 632602

(632602)

Marca:  Takara Bio  | 

Embalagem:  1 System

SISTEMA DE EXPRESSAO E CLONAGEM PARA SGRNAS, 1 KIT

Prazo de Entrega: 30-60 dias corridos

mais informações

Guide-it™ CRISPR/Cas9 System (Red) - Takara Bio - 632602

(632602)

Marca:  Takara Bio  | 

Embalagem:  1 System

SISTEMA DE EXPRESSAO E CLONAGEM PARA SGRNAS, 1 KIT

Prazo de Entrega: 30-60 dias corridos

mais informações

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por: R$ 3.534,38

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Descrição do Produto

Link do produto: http://www.clontech.com/BR/Products/Genome_Editing/CRISPR_Cas9/Plasmid_Systems?sitex=10028:22372:US

 

Cloning and Expression System for sgRNAs

 

The Guide-it CRISPR/Cas9 Systems are kits for the cloning and expression of target single guide RNAs (sgRNAs) for mammalian genome editing using CRISPR/Cas9 technology. The vector in this system simultaneously expresses Cas9 nuclease, a target-specific sgRNA, and an exceptionally bright fluorescent protein for monitoring transfection efficiency and/or for further enriching/isolating transfected cells by flow cytometry (ZsGreen1 and tdTomato versions are available). Generating a plasmid that expresses a sequence-specific sgRNA with this system is simple; a pair of user-provided oligos corresponding to the target genomic sequence of interest are annealed to form a duplex, and the duplexed oligos are inserted into the pre-linearized vector using the included high-efficiency ligation mix. The kit also includes Stellar competent cells to ensure high efficiency transformation.

 

Features

 

  • A single vector expresses your target-specific sgRNA from the human U6 promoter, and Cas9 nuclease and a bright fluorescent marker (either ZsGreen1 or tdTomato) are expressed from a bidirectional CMV promoter
  • tdTomato and ZsGreen1 are exceptionally bright fluorescent proteins, 6x and 2.5x brighter than EGFP, respectively; these markers can be used to monitor transfection efficiency and/or to enrich/isolate transfected cells by flow cytometry
  • Simple plasmid construction—design oligos specific to your target sequence, and clone into the pre-linearized plasmid using the included ligation reagents and high-efficiency competent cells
  • Includes sufficient reagents for construction of 10 different target (sgRNA) expression plasmids

 

Applications

 

  • Cloning and expression of a target single guide RNA (sgRNA) for mammalian genome editing using CRISPR/Cas9 technology

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