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USE SOLVENTE ORGÂNICO MANTENDO O ALTO GRAU DE PUREZA DA SUA AMOSTRA

- Acompanha 50ml de TRI Reagent^TM
- Protocolo completo em 7 minutos!
- Recupera inclusive microRNAs com pelo menos 17 nucleotídeos
- Totalmente livre dos procedimentos de separação de fases e precipitação com álcool.
- Extração a partir de amostras em TRIzol ou outros solventes orgânicos.

The Direct-zol^™ RNA Kits provide a streamlined method for the purification of up to 100 µg (per prep) of high-quality RNAdirectly from samples in TRI Reagent^® or similar.  Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.).

Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis.  The Direct-zol^™ method assures unbiased recovery of small RNAs including miRNA (see below).

The procedure is easy.  Simply apply a prepared sample in TRIReagent^® directly to the Zymo-Spin^™ Column and then bind, wash, and elute the RNA.  No phase separation, precipitation, or post-purification steps are necessary.  The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.).

The entire procedure typically takes only 7 minutes.

Reagent Compatibility: TRIzol®, TRI Reagent®, RNAzol®, QIAzol®, TriPure™, TriSure™, and all other acid-guanidinium-phenol based reagents

*Compatibility is not limited to the samples & reagents listed.

Sample Types: Cells, tissue, plasma, serum, whole blood, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA) 

High-Quality RNA for All Downstream Applications: RT-PCR, miRNA-seq, next-gen sequencing, microarray, Northern blot, and more!