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Estudo de Proteínas

Crosslinkers

BS3 - Thermo Scientific - 21580

BS3 - Thermo Scientific - 21580

(21580)

Marca:  Thermo Scientific  | 

Embalagem:  50 MG

Prazo de Entrega: 30-45 dias corridos

mais informações

BS3 - Thermo Scientific - 21580

(21580)

Marca:  Thermo Scientific  | 

Embalagem:  50 MG

Prazo de Entrega: 30-45 dias corridos

mais informações

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Descrição do Produto

BS3 (bis(sulfosuccinimidyl)suberate)


Thermo Scientific Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.

BS3 contains an amine-reactive N-hydroxysulfosuccinimide (NHS) ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents.

Features of BS3:
Reactive groups: sulfo-NHS ester (both ends)
Reactive towards: amino groups (primary amines)
Amine-reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
Water-soluble; compare with DSS
Membrane-impermeable, allowing for cell surface labeling
High-purity, crystalline reagent can be used to create high-purity crosslinked conjugates

Because it contains the hydrophilic sulfonyl moiety, BS3 crosslinker is soluble up to ~100 mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure. DSS, the non-water soluble analog of BS3 is also available for applications that require a less hydrophilic crosslinker (e.g., to effect intracellular crosslinking). DSS and BS3 have essentially identical crosslinking activity toward primary amines.

Properties of BS3:
Alternative names: Sulfo-DSS
Molecular formula: C16H18N2O14S2Na2
Molecular weight: 572.43
Spacer arm length: 11.4 Å (8 atoms)
CAS number: 82436-77-9
Reactive groups: NHS esters, react with primary amines at pH 7.0-9.0

Applications:
Crosslink cell-surface proteins prior to cell lysis and immunoprecipitation
Identify receptor-ligand interactions by chemical crosslinking
"Fix" protein interactions to allow identification of weak or transient protein interactions
Identify near-neighbor protein interactions
Protein crosslinking to create bioconjugates via single-step reactions
Immobilize proteins onto amine-coated surfaces

BS3 Specifications:
We manufacture BS3 to the highest specifications to produce the most specific bioconjugates, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of BS3 is tested to meet the following minimum specifications:
Purity: > 93% by quantitative NMR (the highest standard for crosslinker purity)
Solubility: > 5.8 mg/mL in DI water, clear solution with no insoluble material

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