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Epigenética

5-hmC

Outros

5-hmC Glucosyltransferase (200 U) - Zymo Research - E2027

5-hmC Glucosyltransferase (200 U) - Zymo Research - E2027

(E2027)

Marca:  Zymo Research  | 

Embalagem:  200 units

ENZIMA 5-HMC GLUCOSYLTRANSFERASE, 200U

Prazo de Entrega: 30-45 dias corridos

mais informações

5-hmC Glucosyltransferase (200 U) - Zymo Research - E2027

(E2027)

Marca:  Zymo Research  | 

Embalagem:  200 units

ENZIMA 5-HMC GLUCOSYLTRANSFERASE, 200U

Prazo de Entrega: 30-45 dias corridos

mais informações

As combinações acima não possuem estoque.

por: R$ 2.018,25

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Descrição do Produto

5-hmC Glucosyltransferase


Specific modification of 5-hydroxymethylcytosine with glucose moiety

 

About 5-hmC Glucosyltransferase

5-hmC Glucosyltransferase from Zymo Research is a highly active enzyme that specifically tags 5-hydroxymethylcytosine in DNA with a glucose moiety yielding glucosyl-5-hydroxymethylcytosine. Glucosylation of 5-hydroxymethylcytosine by 5-hmC Glucosyltransferase can be used for sequence specific and locus specific (see Cat. Nos. D5410 & D5411), as well as global quantification of 5-hydroxymethylcytosine.

 

Applications • Used in method for sequence and locus specfic detection of 5-hydroxymethylcytosine within DNA 
• Global quantification of 5-hydroxymethylcytosine
Storage 5-hmC Glucosyltransferase is guaranteed for 12 months at -20°C. Long term storage at -80°C is recommended. Avoid multiple freeze thawing.
Unit Definition One unit (1 U) is defined as the amount of enzyme needed to “protect” 1µg of 5hmC DNA Standard [D5405-3] from GlaI digestion via glucosylation in a reaction incubate at 30°C for 1 hour.
Standard Reaction Setup
Reagent Volume
DNA [100ng/µl] 10 µl
10x 5hmC GT Reaction Buffer 5 µl
10xUDPG [1mM] 5 µl
5hmC GT Enzyme 2 µl
ddH2O 28 µl
Total Volume 50 µl
Enzyme Concentration 2 units/µl

 

Link do Produto: http://www.zymoresearch.com/dna/enzymes/dna-modifying-enzymes/5-hmc-glucosyltransferase

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